Mitochondria-Localized Glutamic Acid-Rich Protein (MGARP) Gene Transcription Is Regulated by Sp1

Background: Mitochondria-localized glutamic acid-rich protein (MGARP) is a novel mitochondrial transmembrane protein expressed mainly in steroidogenic tissues and in the visual system. Previous studies showed that MGARP functions in hormone biosynthesis and its expression is modulated by the HPG axis. Methodology/principal findings: By bioinformatics, we identified two characteristic GC-rich motifs that are located proximal to the transcription start site (TSS) of MGARP, and each contains two Specificity protein 1 (Sp1) binding elements. We then determined that the −3 kb proximal MGARP promoter is activated in a Sp1-dependent manner using reporter assays and knockdown of Sp1 led to decreased expression of endogenous MGARP messages. We also demonstrated that one of the two GC-rich motifs, GC-Box1, harbors prominent promoter activity mediated by Sp1, and that it requires both GC boxes for full transcriptional activation. These findings suggest a dominant role for these GC boxes and Sp1 in activating the MGARP promoter through a synergistic mechanism. Consistently, the results of an Electrophoretic Mobility Gel Shift Assay (EMSA) and Chromatin Immunoprecipitation (ChIP) confirmed that Sp1 specifically interacts with the GC-rich region. We further found that estrogen receptor α (ERα), a known Sp1 co-activator, could potentiate GC-boxes containing MGARP promoter activity and this effect is mediated by Sp1. Knockdown of Sp1 significantly diminished the MGARP promoter transactivation and the expression of endogenous MGARP mediated by both Sp1 and ERα. Conclusions/significance: The present study identified a proximal core sequence in the MGARP promoter that is composed of two enriched Sp1 binding motifs and established Sp1 as one major MGARP transactivator whose functions are synergistic with ERα, providing a novel understanding of the mechanisms of MGARP gene transcriptional regulation

Quantum Attacks on Lai-Massey Structure

Aaram Yun et al. considered that Lai-Massey structure has the same security as Feistel structure. However, Luo et al. showed that 3-round Lai-Massey structure can resist quantum attacks of Simon\u27s algorithm, which is different from Feistel structure. We give quantum attacks against a typical Lai-Massey structure. The result shows that there exists a quantum CPA distinguisher against 3-round Lai-Massey structure and a quantum CCA distinguisher against 4-round Lai-Massey Structure, which is the same as Feistel structure. We extend the attack on Lai-Massey structure to quasi-Feistel structure. We show that if the combiner of quasi-Feistel structure is linear, there exists a quantum CPA distinguisher against 3-round balanced quasi-Feistel structure and a quantum CCA distinguisher against 4-round balanced quasi-Feistel Structure

Prognostic significance of circulating tumor cell measurement in the peripheral blood of patients with nasopharyngeal carcinoma

Objective: Nasopharyngeal Carcinoma (NPC) is lethal cancer. Typically, relapse and metastasis are the outcomes of most patients. Against this backdrop, this study aimed to investigate the correlation between Circulating Tumor Cell (CTC) profiles and clinicopathological features in patients with NPC. Patients and methods: A total of 119 blood samples from 79 patients were collected from patients with NPC during treatment. CanPatrolTM CTC enrichment and RNA In Situ Hybridization (RNA-ISH) were used to characterize CTCs, including epithelial, Mesenchymal (MCTCs), and epithelial/mesenchymal mixed types according to their surface markers. Results: The number of CTCs and MCTCs in the pre-treatment group was significantly higher than that in the post-treatment group (p < 0.05). The total number of CTCs and MCTCs cell numbers was significant correlation with Tumor-Node-Metastasis (TNM) staging (p < 0.05), Progression-Free Survival (PFS), and Overall Survival (OS). The PFS of patients with > 7 CTCs or > 5 MCTCs per 5 mL blood was significantly shorter PFS than those patients with ≤ 7 CTCs or ≤ 5 MCTCs (p < 0.05). Patients treated with targeted therapy combined with chemoradiotherapy had poorer PFS and OS rates than those treated with chemoradiotherapy (p < 0.05). The Kaplan-Meier survival analysis also demonstrated that patients with changes in CTC > 4 were strongly associated with PFS and OS rates (p < 0.05). Conclusion: CTC and MCTC number detection in patients with NPC is a useful biomarker for predicting patient progress. Patients with more than 7 CTCs or 5 MCTCs in 5 mL of blood had shorter PFS and OS rates. CTC and MCTC count changes were also significantly associated with the patient's therapy

Small Stretch Problem of the DCT Scheme and How to Fix it

DCT is a beyond-birthday-bound~(BBB) deterministic authenticated encryption~(DAE) mode proposed by Forler et al. in ACISP 2016, ensuring integrity by redundancy. The instantiation scheme of DCT employs the BRW polynomial, which is more efficient than the usual polynomial function in GCM by reducing half of the multiplication operations. However, we show that DCT suffers from a small stretch problem similar to GCM. When the stretch length $\tau$ is small, choosing a special $m$-block message, we can reduce the number of queries required by a successful forgery to $\mathcal{O}(2^{\tau}/m)$. We emphasize that this attack efficiently balances space and time complexity, but does not contradict the security bounds of DCT. Finally, we propose an improved scheme named Robust DCT~(RDCT) with a minor change to DCT, which improves the security when $\tau$ is small and makes it resist the above attack

Iterative Frequency Domain Equalization for MIMO-GFDM Systems

This paper proposes a new iterative frequency domain equalization (FDE) algorithm for multiple-input multiple-output (MIMO)-frequency division multiplexing (GFDM) systems. This new FDE scheme is capable of enhancing the system fidelity by considering the complete frequency-domain second order description of the received signal. In addition, a new nulling filter design is also proposed for MIMO-GFDM systems to remove the residual interference, which further improves the system fidelity compared with the traditional scheme. Simulation results are presented to verify the effiectiveness and efficiency of the proposed FDE algorithm

The pregnancy-related anxiety characteristics in women with gestational diabetes mellitus: why should we care?

Abstract Background Gestational diabetes mellitus (GDM) is very commonly-seen in clinical settings, and GDM patients may have higher levels of anxiety. It’s necessary to evaluate the anxiety level and potentially influencing factors in patients with GDM, to provide insights for the management of anxiety of GDM patients. Methods Patients with GDM treated in our hospital from May, 2018 to May, 2020 were included. We evaluated the characteristics of patients and the scores of pregnancy-related anxiety scale for anxiety level, vulnerable personality style questionnaire (VPSQ) for personality, general self-efficacy scale (GSES) for self-efficacy, social support rating scale (SSRS) for social support level. Logistic regression analyses were conducted to identify the potential influencing factors of anxiety in GDM patients. Results A total of 386 GDM patients were included, the incidence of anxiety in patients with GDM was 59.07%. Anxiety was positively correlated with the susceptible personality (r = 0.604, p = 0.023), and it was negatively correlated with self-efficacy and social support (r = -0.586 and -0.598 respectively, all p < 0.05). The education level, monthly income, abnormal pregnancy (miscarriage, premature rupture of membranes) and cesarean section history and first pregnancy were the independent influencing factors for the anxiety in the patients with GDM (all p < 0.05). Conclusions The anxiety of GDM patients is very common, early care and interventions are warranted for those patients with abnormal pregnancy and cesarean section history, first pregnancy, lower education level, and less monthly income

Current Progress in Bioactive Ceramic Scaffolds for Bone Repair and Regeneration

Bioactive ceramics have received great attention in the past decades owing to their success in stimulating cell proliferation, differentiation and bone tissue regeneration. They can react and form chemical bonds with cells and tissues in human body. This paper provides a comprehensive review of the application of bioactive ceramics for bone repair and regeneration. The review systematically summarizes the types and characters of bioactive ceramics, the fabrication methods for nanostructure and hierarchically porous structure, typical toughness methods for ceramic scaffold and corresponding mechanisms such as fiber toughness, whisker toughness and particle toughness. Moreover, greater insights into the mechanisms of interaction between ceramics and cells are provided, as well as the development of ceramic-based composite materials. The development and challenges of bioactive ceramics are also discussed from the perspective of bone repair and regeneration